Boster Immunoleader

ELISA/assay

Mouse CD22 PicoKine ELISA Kit

EK1303

1 x96 well plate

369 USD

Mouse CD22 PicoKine ELISA Kit

EK1303

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CD22

NSO, S22-R702

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.86; Standard deviation: 0.099; CV(%): 5.3 Sample 2: n=16; Mean(ng/ml): 4.32; Standard deviation: 0.207; CV(%): 4.8 Sample 3: n=16; Mean(ng/ml): 7.35; Standard deviation: 0.309; CV(%): 4.2

Sample 1: n=24; Mean(ng/ml): 2.54; Standard deviation: 0.17; CV(%): 6.7 Sample 2: n=24; Mean(ng/ml): 5.13; Standard deviation: 0.313; CV(%): 6.1 Sample 3: n=24; Mean(ng/ml): 7.44; Standard deviation: 0.417; CV(%): 5.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CD22 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CD22 antibody 1 ST0000-10 Lyophilized recombinant mouse CD22 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CD22 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors(BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.

CD22

B-cell receptor CD22

CD22 molecule

B cell receptor CD22 precursor B lymphocyte cell adhesion molecule B-cell receptor CD22 B-lymphocyte cell adhesion molecule BL CAM BL-CAM BLCAM CD 22 CD22 CD22 antigen CD22 molecule CD22 protein CD22_HUMAN Lectin 2 Leu14 Lyb8 MGC130020 sialic acid binding Ig like lectin 2 Sialic acid binding immunoglobulin like lectin 2 Sialic acid-binding Ig-like lectin 2 SIGLEC 2 Siglec-2 SIGLEC2 T cell surface antigen Leu 14 T-cell surface antigen Leu-14

P35329