Boster Immunoleader

ELISA/assay

Rat HGF PicoKine ELISA Kit

EK1301

1 x96 well plate

369 USD

Rat HGF PicoKine ELISA Kit

EK1301

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat HGF

NSO, Q33-R495(alpha) & V496-L728(beta)

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1765; Standard deviation: 74.13; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 3081; Standard deviation: 141.7; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 6143; Standard deviation: 344; CV(%): 5.6

Sample 1: n=24; Mean(pg/ml): 1860; Standard deviation: 126.5; CV(%): 6.8 Sample 2: n=24; Mean(pg/ml): 3542; Standard deviation: 180.6; CV(%): 5.1 Sample 3: n=24; Mean(pg/ml): 6496; Standard deviation: 487.2; CV(%): 7.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat HGF in cell culture supernates, serum and plasma(EDTA, citrate).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat HGF antibody 1 ST0000-10 Lyophilized recombinant rat HGF standard 10ng/tubex2 AR1107 Biotinylated anti- rat HGF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.

HGF

Hepatocyte growth factor

hepatocyte growth factor (hepapoietin A; scatter factor)

DFNB39 F TCF Fibroblast derived tumor cytotoxic factor Hepapoietin A Hepatocyte growth factor Hepatocyte growth factor (hepapoietin A scatter factor Hepatocyte growth factor beta chain Hepatopoietin-A Hgf HGF_HUMAN HGFB HPTA Lung fibroblast derived mitogen Scatter factor SF

P17945