Boster Immunoleader

ELISA/assay

Rat ADAM12 PicoKine ELISA Kit

EK1293

1 x96 well plate

369 USD

Rat ADAM12 PicoKine ELISA Kit

EK1293

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat ADAM12

CHO, R29-S513

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.46; Standard deviation: 0.066; CV(%): 4.5 Sample 2: n=16; Mean(ng/ml): 3.65; Standard deviation: 0.139; CV(%): 3.8 Sample 3: n=16; Mean(ng/ml): 6.89; Standard deviation: 0.234; CV(%): 3.4

Sample 1: n=24; Mean(ng/ml): 1.55; Standard deviation: 0.088; CV(%): 5.7 Sample 2: n=24; Mean(ng/ml): 3.81; Standard deviation: 0.175; CV(%): 4.6 Sample 3: n=24; Mean(ng/ml): 6.92; Standard deviation: 0.324; CV(%): 4.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat ADAM12 in cell culture supernates, serum and plasma(heparin).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat ADAM12 antibody 1 ST0000-10 Lyophilized recombinant rat ADAM12 standard 10ng/tubex2 AR1107 Biotinylated anti- rat ADAM12 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

ADAM12

Disintegrin and metalloproteinase domain-containing protein 12

ADAM metallopeptidase domain 12

ADA12_HUMAN ADAM 12 ADAM metallopeptidase domain 12 ADAM12 Disintegrin and metalloproteinase domain-containing protein 12 MCMP MCMPMltna Meltrin-alpha MLTN MLTNA

F1M9Z9