Boster Immunoleader

ELISA/assay

Human Granulysin PicoKine ELISA Kit

EK1280

1 x96 well plate

369 USD

Human Granulysin PicoKine ELISA Kit

EK1280

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Granulysin

NSO, M1-L145

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 355; Standard deviation: 20.59; CV(%): 5.8 Sample 2: n=16; Mean(pg/ml): 862; Standard deviation: 38.79; CV(%): 4.5 Sample 3: n=16; Mean(pg/ml): 1513; Standard deviation: 65; CV(%): 4.3

Sample 1: n=24; Mean(pg/ml): 324; Standard deviation: 26.5; CV(%): 8.2 Sample 2: n=24; Mean(pg/ml): 765; Standard deviation: 55.85; CV(%): 7.3 Sample 3: n=24; Mean(pg/ml): 1322; Standard deviation: 89.8; CV(%): 6.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Granulysin in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Granulysin antibody 1 ST0000-10 Lyophilized recombinant human Granulysin standard 10ng/tubex2 AR1107 Biotinylated anti- human Granulysin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Granulysin is a substance released by cytotoxic T cells(CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein(SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.

GNLY

Granulysin

granulysin

519 D2S69E GNLY GNLY_HUMAN Granulysin LAG 2 LAG2 Lymphocyte activation gene 2 Lymphokine LAG 2 Lymphokine LAG-2 NKG 5 NKG5 Protein NKG5 T cell activation protein 519 T lymphocyte activation gene 519 T-cell activation protein 519 TLA519

P22749