Boster Immunoleader

ELISA/assay

Mouse Prostasin PicoKine ELISA Kit

EK1273

1 x96 well plate

369 USD

Mouse Prostasin PicoKine ELISA Kit

EK1273

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Prostasin

NSO, A30-Q289

1 x96 well plate

0.78ng/ml-50ng/ml

<50pg/ml

Sample 1: n=16; Mean(ng/ml): 1.45; Standard deviation: 0.08; CV(%): 5.6 Sample 2: n=16; Mean(ng/ml): 25.2; Standard deviation: 1.06; CV(%): 4.2 Sample 3: n=16; Mean(ng/ml): 38.1; Standard deviation: 2.02; CV(%): 5.3

Sample 1: n=24; Mean(ng/ml): 2.64; Standard deviation: 0.19; CV(%): 7.2 Sample 2: n=24; Mean(ng/ml): 18.5; Standard deviation: 1.18; CV(%): 6.4 Sample 3: n=24; Mean(ng/ml): 40.7; Standard deviation: 3.5; CV(%): 8.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse Prostasin in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse Prostasin antibody 1 ST0000-50 Lyophilized recombinant mouse Prostasin standard 50ng/tubex2 AR1107 Biotinylated anti- mouse Prostasin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

PRSS8

Prostasin

protease, serine, 8

2410039E18Rik AI313909 C79772 CAP1 Channel Activating Protease 1 mCAP1 Prostasin Prostasin heavy chain protease, serine, 8 Prss8 PRSS8_HUMAN Serine protease 8

Q9ESD1