Boster Immunoleader

ELISA/assay

Human SPINK1/TATI PicoKine ELISA Kit

EK1241

1 x96 well plate

369 USD

Human SPINK1/TATI PicoKine ELISA Kit

EK1241

369 USD

Sandwich ELISA kit for Quantitative Detection of Human SPINK1/TATI

E.coli, D24-C79

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 1.3; Standard deviation: 0.052; CV(%): 4 Sample 2: n=16; Mean(ng/ml): 2.7; Standard deviation: 0.138; CV(%): 5.1 Sample 3: n=16; Mean(ng/ml): 4.8; Standard deviation: 0.274; CV(%): 5.7

Sample 1: n=24; Mean(ng/ml): 1.6; Standard deviation: 0.12; CV(%): 7.5 Sample 2: n=24; Mean(ng/ml): 2.4; Standard deviation: 0.13; CV(%): 5.4 Sample 3: n=24; Mean(ng/ml): 5.1; Standard deviation: 0.332; CV(%): 6.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human SPINK1 in cell culture supernates, serum and plasma(heparin).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human SPINK1 antibody 1 ST0000-10 Lyophilized recombinant human SPINK1 standard 10ng/tubex2 AR1107 Biotinylated anti- human SPINK1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

SPINK1

Pancreatic secretory trypsin inhibitor

serine peptidase inhibitor, Kazal type 1

ISK1_HUMAN Pancreatic secretory trypsin inhibitor Pancreatic serine protease inhibitor Kazal PCTT PSTI Serine protease inhibitor Kazal type 1 Serine protease inhibitor Kazal-type 1 Spink 3 SPINK1 Spink3 TaTI TCP Tumor associated trypsin inhibitor Tumor-associated trypsin inhibitor

P00995