Boster Immunoleader

ELISA/assay

Human Midkine PicoKine ELISA Kit

EK1235

1 x96 well plate

369 USD

Human Midkine PicoKine ELISA Kit

EK1235

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Midkine

E.coli, K23-D143

1 x96 well plate

78pg/ml-5000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 465; Standard deviation: 21.4; CV(%): 4.6 Sample 2: n=16; Mean(pg/ml): 1616; Standard deviation: 88.88; CV(%): 5.5 Sample 3: n=16; Mean(pg/ml): 2823; Standard deviation: 169.4; CV(%): 6

Sample 1: n=24; Mean(pg/ml): 582; Standard deviation: 30.85; CV(%): 5.3 Sample 2: n=24; Mean(pg/ml): 1828; Standard deviation: 122.5; CV(%): 6.7 Sample 3: n=24; Mean(pg/ml): 3012; Standard deviation: 220; CV(%):7.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Midkine in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti-human Midkine antibody 1 ST0000-10 Lyophilized recombinant human Midkine standard 10ng/tubex2 AR1107 Biotinylated anti- human Midkine antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Midkine(MK or MDK) also known as neurite growth-promoting factor 2(NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPζ), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase(ALK) and syndecans is considered to be its receptor.

MDK

Midkine

midkine (neurite growth-promoting factor 2)

Amphiregulin-associated protein ARAP Mdk Midgestation and kidney protein Midkine MK MK_HUMAN Neurite outgrowth-promoting factor 2 Neurite outgrowth-promoting protein

P21741