Boster Immunoleader

ELISA/assay

Human TFF1 PicoKine ELISA Kit

EK1232

1 x96 well plate

369 USD

Human TFF1 PicoKine ELISA Kit

EK1232

369 USD

Sandwich ELISA kit for Quantitative Detection of Human TFF1

E.coli, E25-F84

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 92; Standard deviation: 3.5; CV(%): 3.8 Sample 2: n=16; Mean(pg/ml): 332; Standard deviation: 14.94; CV(%): 4.5 Sample 3: n=16; Mean(pg/ml): 585; Standard deviation: 32.76; CV(%): 5.6

Sample 1: n=24; Mean(pg/ml): 103; Standard deviation: 4.84; CV(%): 4.7 Sample 2: n=24; Mean(pg/ml): 310; Standard deviation: 16.43; CV(%): 5.3 Sample 3: n=24; Mean(pg/ml): 605; Standard deviation: 38.72; CV(%): 6.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human TFF1 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human TFF1 antibody 1 ST0000-1 Lyophilized recombinant human TFF1 standard 1ng/tubex2 AR1107 Biotinylated anti- human TFF1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

TFF1

Trefoil factor 1

trefoil factor 1

BCEI Breast cancer estrogen inducible protein Breast cancer estrogen inducible sequence Breast cancer estrogen-inducible protein D21S21 Gastrointestinal trefoil protein Gastrointestinal trefoil protein pS2 hP1.A HP1A HPS 2 HPS2 pNR 2 PNR-2 pNR2 Polypeptide P1.A Protein pS2 PS 2 pS2 pS2 protein TFF 1 TFF1 TFF1_HUMAN Trefoil factor 1

P04155