product summary
request information :
company name :
Boster Immunoleader
product type :
ELISA/assay
product name :
Mouse CD13/Aminopeptidase N PicoKine ELISA Kit
catalog :
EK1222
quantity :
1 x96 well plate
price :
369 USD
product information
Product Name :
Mouse CD13/Aminopeptidase N PicoKine ELISA Kit
Catalog Number :
EK1222
Price :
369 USD
Description short :
Sandwich ELISA kit for Quantitative Detection of Mouse CD13/Aminopeptidase N
immunogen :
NSO, K69-S966
Pack Size :
1 x96 well plate
Assay Range :
312pg/ml-20,000pg/ml
Sensitivity :
<10pg/ml
Intra-Assay Precision :
Sample 1: n=16; Mean(ng/ml): 3.15; Standard deviation: 0.164; CV(%): 5.2 Sample 2: n=16; Mean(ng/ml): 6.64; Standard deviation: 0.372; CV(%): 5.6 Sample 3: n=16; Mean(ng/ml): 13.09; Standard deviation: 0.785; CV(%): 6.0
Inter-Assay Precision :
Sample 1: n=24; Mean(ng/ml): 3.36; Standard deviation: 0.218; CV(%): 6.5 Sample 2: n=24; Mean(ng/ml): 6.97; Standard deviation: 0.474; CV(%): 6.8 Sample 3: n=24; Mean(ng/ml): 14.15; Standard deviation: 1.02; CV(%): 7.2
Cross Reactivity :
There is no detectable cross-reactivity with other relevant proteins.
Storage :
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Sample Type :
This kit is used for quantitative detection of Mouse CD13 in cell culture supernates, serum and plasma(heparin, EDTA).
Reactivity :
Mouse
Kit Components :
Catalog number Description Quantity - 96-well plate precoated with anti- Mouse CD13 antibody 1 ST0000-20 Lyophilized recombinant mouse CD13 standard 20ng/tubex2 AR1107 Biotinylated anti- mouse CD13 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml
Background :
Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.
Gene Name :
ANPEP
Protein Name :
Aminopeptidase N
Gene Full Name :
alanyl (membrane) aminopeptidase
Synonyms :
Alanyl (membrane) aminopeptidase Alanyl aminopeptidase Alanyl membrane aminopeptidase Aminopeptidase M Aminopeptidase N AMPN_HUMAN ANPEP AP M AP N AP-M AP-N APN CD 13 CD13 CD13 antigen gp150 hAPN Lap 1 Lap1 Microsomal aminopeptidase Myeloid plasma membrane glycoprotein CD13 p150 PEPN
Uniprot IDs :
P97449
company information
Boster Immunoleader
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
www.bosterbio.com
925.485.4527
headquarters: USA
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