Boster Immunoleader

ELISA/assay

Human soluble CD5 PicoKine ELISA Kit

EK1220

1 x96 well plate

369 USD

Human soluble CD5 PicoKine ELISA Kit

EK1220

369 USD

Sandwich ELISA kit for Quantitative Detection of Human soluble CD5

NSO, R25-N371

1 x96 well plate

93.7pg/ml-6000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.37; Standard deviation: 0.023; CV(%): 6.2 Sample 2: n=16; Mean(ng/ml): 0.85; Standard deviation: 0.041; CV(%): 4.8 Sample 3: n=16; Mean(ng/ml): 4.27; Standard deviation: 0.167; CV(%): 3.9

Sample 1: n=24; Mean(ng/ml): 0.38; Standard deviation: 0.031; CV(%): 8.2 Sample 2: n=24; Mean(ng/ml): 0.92; Standard deviation: 0.699; CV(%): 7.6 Sample 3: n=24; Mean(ng/ml): 4.31; Standard deviation: 0.311; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD5 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human CD5 antibody 1 ST0000-10 Lyophilized recombinant human CD5 standard 10ng/tubex2 AR1107 Biotinylated anti- human CD5 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.

CD5

T-cell surface glycoprotein CD5

CD5 molecule

CD 5 CD5 CD5 antigen (p56 62) CD5 antigen CD5 molecule CD5_HUMAN LEU 1 LEU1 Ly12 LyA Lymphocyte Antigen CD5 Lymphocyte antigen T1/Leu 1 Lymphocyte antigen T1/Leu-1 Lymphocyte glycoprotein T1/Leu1 OTTHUMP00000236973 p56 62 T cell surface glycoprotein CD5 T-cell surface glycoprotein CD5 T1

P06127