Boster Immunoleader

ELISA/assay

Rat MIP-1alpha/CCL3 PicoKine ELISA Kit

EK1219

1 x96 well plate

369 USD

Rat MIP-1alpha/CCL3 PicoKine ELISA Kit

EK1219

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat MIP-1alpha/CCL3

E.coli, A24-A92

1 x96 well plate

7.8pg/ml-500pg/ml

<1pg/ml

Sample 1: n=16; Mean(pg/ml): 53; Standard deviation: 2.76; CV(%): 5.2 Sample 2: n=16; Mean(pg/ml): 177; Standard deviation: 11.86; CV(%): 6.7 Sample 3: n=16; Mean(pg/ml): 293; Standard deviation: 13.2; CV(%): 4.5

Sample 1: n=24; Mean(pg/ml): 65; Standard deviation: 4.1; CV(%): 6.3 Sample 2: n=24; Mean(pg/ml): 192; Standard deviation: 14.6; CV(%): 7.6 Sample 3: n=24; Mean(pg/ml): 317; Standard deviation: 18.4; CV(%): 5.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat MIP-1alpha in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat MIP-1alpha antibody 1 ST0000-10 Lyophilized recombinant rat MIP-1alpha standard 10ng/tubex2 AR1107 Biotinylated anti- rat MIP-1alpha antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.

CCL3

C-C motif chemokine 3

chemokine (C-C motif) ligand 3

C C motif chemokine 3 CCL3 CCL3_HUMAN Chemokine (C C motif) ligand 3 Chemokine ligand 3 G0/G1 switch regulatory protein 19 1 G0/G1 switch regulatory protein 19-1 G0S19 1 G0S19 1 protein Heparin binding chemotaxis protein L2G25B LD78-alpha(4-69) LD78ALPHA Macrophage inflammatory protein 1 alpha Macrophage inflammatory protein 1-alpha MIP 1 alpha MIP-1-alpha MIP-1-alpha(4-69) MIP1A PAT 464.1 SCYA 3 SCYA3 SIS alpha SIS beta SIS-beta Small inducible cytokine A3 Small-inducible cytokine A3 Tonsillar lymphocyte LD78 alpha protein

P50229