Boster Immunoleader

ELISA/assay

Human LAP(TGF-beta1) PicoKine ELISA Kit

EK1218

1 x96 well plate

369 USD

Human LAP(TGF-beta1) PicoKine ELISA Kit

EK1218

369 USD

Sandwich ELISA kit for Quantitative Detection of Human LAP(TGF-beta1)

sf21, L30-R278

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 347; Standard deviation: 13.88; CV(%): 4 Sample 2: n=16; Mean(pg/ml): 1638; Standard deviation: 85.18; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 2586; Standard deviation: 163; CV(%): 6.3

Sample 1: n=24; Mean(pg/ml): 511; Standard deviation: 29.64; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 1825; Standard deviation: 100.4; CV(%): 5.5 Sample 3: n=24; Mean(pg/ml): 3120; Standard deviation: 246.5; CV(%): 7.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human LAP(TGF-beta1) in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human LAP(TGF-beta1) antibody 1 ST0000-10 Lyophilized recombinant human LAP(TGF-beta1) standard 10ng/tubex2 AR1107 Biotinylated anti- human LAP(TGF-beta1) antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.

TGFB1

Transforming growth factor beta-1

transforming growth factor, beta 1

Camurati-Engelmann disease CED DPD1 LAP Latency-associated peptide TGF beta 1 protein TGF-beta-1 TGFB tgfb1 TGFB1_HUMAN TGFbeta Transforming growth factor beta 1

P01137