Boster Immunoleader

ELISA/assay

Human SPARC PicoKine ELISA Kit

EK1210

1 x96 well plate

369 USD

Human SPARC PicoKine ELISA Kit

EK1210

369 USD

Sandwich ELISA kit for Quantitative Detection of Human SPARC

NSO, A18-I303

1 x96 well plate

0.78ng/ml-50ng/ml

<20pg/ml

Sample 1: n=16; Mean(ng/ml): 7; Standard deviation: 0.357; CV(%): 5.1 Sample 2: n=16; Mean(ng/ml): 19.5; Standard deviation: 0.819; CV(%): 4.2 Sample 3: n=16; Mean(ng/ml): 32.2; Standard deviation: 1.26; CV(%): 4.8

Sample 1: n=24; Mean(ng/ml): 10.1; Standard deviation: 0.596; CV(%): 5.9 Sample 2: n=24; Mean(ng/ml): 24.5; Standard deviation: 1.568; CV(%): 7.4 Sample 3: n=24; Mean(ng/ml): 38.4; Standard deviation: 2.04; CV(%): 5.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human SPARC in cell culture supernates, serum and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human SPARC antibody 1 ST0000-50 Lyophilized recombinant human SPARC standard 50ng/tubex2 AR1107 Biotinylated anti- human SPARC antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

SPARC(secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I), 2) a cysteine- rich(domain II), 3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

SPARC

SPARC

secreted protein, acidic, cysteine-rich (osteonectin)

AA517111 Basement membrane protein 40 Basement-membrane protein 40 BM 40 BM-40 BM40 Cysteine rich protein MGC128090 ON Osteonectin Secreted acidic cystein rich glycoprotein Secreted protein acidic and rich in cysteine Secreted protein acidic cysteine rich (osteonectin) Secreted protein acidic cysteine rich SPARC SPRC SPRC_HUMAN

P09486