Boster Immunoleader

ELISA/assay

Human Cystatin B PicoKine ELISA Kit

EK1205

1 x96 well plate

369 USD

Human Cystatin B PicoKine ELISA Kit

EK1205

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Cystatin B

E.coli, M2-F98

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 421; Standard deviation: 26.1; CV(%): 6.2 Sample 2: n=16; Mean(pg/ml): 1169; Standard deviation: 67.8; CV(%): 5.8 Sample 3: n=16; Mean(pg/ml): 2311; Standard deviation: 140.97; CV(%): 6.1

Sample 1: n=24; Mean(pg/ml): 428; Standard deviation: 30.82; CV(%): 7.2 Sample 2: n=24; Mean(pg/ml): 1207; Standard deviation: 82.08; CV(%): 6.8 Sample 3: n=24; Mean(pg/ml): 2365; Standard deviation: 165.6; CV(%): 7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Cystatin B in cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Cystatin B antibody 1 ST0000-10 Lyophilized recombinant human Cystatin B standard 10ng/tubex2 AR1107 Biotinylated anti- human Cystatin B antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Cystatin B(CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.

CSTB

Cystatin-B

cystatin B (stefin B)

CHROW21 CPI B CPI-B CST6 CSTB Cystatin B Cystatin-B CYTB CYTB_HUMAN EPM1 EPM1A Liver thiol proteinase inhibitor PME Stefin-B STFB ULD

P04080