Boster Immunoleader

ELISA/assay

Human WISP1/CCN4 PicoKine ELISA Kit

EK1204

1 x96 well plate

369 USD

Human WISP1/CCN4 PicoKine ELISA Kit

EK1204

369 USD

Sandwich ELISA kit for Quantitative Detection of Human WISP1/CCN4

NSO, T23-N367

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 236; Standard deviation: 7.55; CV(%): 3.2 Sample 2: n=16; Mean(pg/ml): 1087; Standard deviation: 46.7; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 1826; Standard deviation: 89.5; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 352; Standard deviation: 16.2; CV(%): 4.6 Sample 2: n=24; Mean(pg/ml): 1240; Standard deviation: 64.5; CV(%): 5.2 Sample 3: n=24; Mean(pg/ml): 1910; Standard deviation: 128; CV(%): 6.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human WISP1 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human WISP1 antibody 1 ST0000-10 Lyophilized recombinant human WISP1 standard 10ng/tubex2 AR1107 Biotinylated anti- human WISP1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

WISP1

WNT1-inducible-signaling pathway protein 1

WNT1 inducible signaling pathway protein 1

CCN family member 4 CCN4 WISP1 WISP1c WISP1i WISP1tc Wnt 1 inducible signaling pathway protein 1 Wnt-1 induced secreted protein WNT1 induced secreted protein 1 WNT1 inducible signaling pathway protein 1 WNT1 inducible-signaling pathway protein 1

O95388