Boster Immunoleader

ELISA/assay

Human MFGE8/Lactadherin PicoKine ELISA Kit

EK1201

1 x96 well plate

369 USD

Human MFGE8/Lactadherin PicoKine ELISA Kit

EK1201

369 USD

Sandwich ELISA kit for Quantitative Detection of Human MFGE8/Lactadherin

NSO, L24-C387

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 669; Standard deviation: 42.82; CV(%): 6.4 Sample 2: n=16; Mean(pg/ml): 1295; Standard deviation: 58.3; CV(%): 4.5 Sample 3: n=16; Mean(pg/ml): 2234; Standard deviation: 116.2; CV(%): 5.2

Sample 1: n=24; Mean(pg/ml): 724; Standard deviation: 56.5; CV(%): 7.8 Sample 2: n=24; Mean(pg/ml): 1443; Standard deviation: 86.58; CV(%): 6 Sample 3: n=24; Mean(pg/ml): 2458; Standard deviation: 135.2; CV(%): 5.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MFGE8 in cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MFGE8 antibody 1 ST0000-10 Lyophilized recombinant human MFGE8 standard 10ng/tubex2 AR1107 Biotinylated anti- human MFGE8 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

MFGE8

Lactadherin

milk fat globule-EGF factor 8 protein

BA46 Breast epithelial antigen BA46 Contains Medin EDIL1 HMFG hP47 HsT19888 Lactadherin Lactadherin [Precursor] Medin MFG E8 MFG-E8 MFGE8 MFGM MFGM_HUMAN Milk fat globule EGF factor 8 Milk fat globule EGF factor 8 protein Milk fat globule-EGF factor 8 O acetyl disialoganglioside synthase OAcGD3S SED1 SPAG10 Sperm associated antigen 10 Sperm surface protein hP47

Q08431