Boster Immunoleader

ELISA/assay

Mouse ALCAM PicoKine ELISA Kit

EK1184

1 x96 well plate

369 USD

Mouse ALCAM PicoKine ELISA Kit

EK1184

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse ALCAM

NSO, W28-K527

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 0.57; Standard deviation: 0.029; CV(%): 5.1 Sample 2: n=16; Mean(ng/ml): 2.33; Standard deviation: 0.086; CV(%): 3.7 Sample 3: n=16; Mean(ng/ml): 4.25; Standard deviation: 0.136; CV(%): 3.2

Sample 1: n=24; Mean(ng/ml): 0.68; Standard deviation: 0.058; CV(%): 8.6 Sample 2: n=24; Mean(ng/ml): 2.74; Standard deviation: 0.17; CV(%): 6.2 Sample 3: n=24; Mean(ng/ml): 4.61; Standard deviation: 0.244; CV(%): 5.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse ALCAM in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse ALCAM antibody 1 ST0000-10 Lyophilized recombinant mouse ALCAM standard 10ng/tube×2 AR1107 Biotinylated anti- mouse ALCAM antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

ALCAM

CD166 antigen

activated leukocyte cell adhesion molecule

Activated leukocyte cell adhesion molecule ALCAM ALCAM protein CD 166 CD166 CD166 antigen CD166_HUMAN FLJ3851 FLJ38514 MEMD MGC71733

Q61490