Boster Immunoleader

ELISA/assay

Human Granzyme A PicoKine ELISA Kit

EK1162

1 x96 well plate

369 USD

Human Granzyme A PicoKine ELISA Kit

EK1162

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Granzyme A

NSO, I29-V262

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 89.5; Standard deviation: 4.65; CV(%): 5.2 Sample 2: n=16; Mean(pg/ml): 376; Standard deviation: 16.17; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 525; Standard deviation: 23.10; CV(%): 4.4

Sample 1: n=24; Mean(pg/ml): 97.6; Standard deviation: 7.32; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 368; Standard deviation: 25.39; CV(%): 6.9 Sample 3: n=24; Mean(pg/ml): 567; Standard deviation: 44.23; CV(%): 7.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Granzyme A in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Granzyme A antibody 1 ST0000-10 Lyophilized recombinant human Granzyme A standard 10ng/tube×2 AR1107 Biotinylated anti- human Granzyme A antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A.

GZMA

Granzyme A

granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)

CTL tryptase CTLA3 Cytolytic t cell and natural killer cell specific trypsin like serine protease Cytotoxic T lymphocyte associated serine esterase 3 Cytotoxic T lymphocyte proteinase 1 Cytotoxic T-lymphocyte proteinase 1 Fragmentin 1 Fragmentin-1 GRAA_HUMAN Granzyme 1 cytotoxic T lymphocyte associated serine esterase 3 Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease) Granzyme A (granzyme 1, cytotoxic T lymphocyte associated serine esterase 3) Granzyme A Granzyme A precursor Granzyme-1 GZMA H factor Hanukah factor serine protease Hanukkah factor HF HFSP TSP1

P12544