Boster Immunoleader

ELISA/assay

Human Nesfatin-1 PicoKine ELISA Kit

EK1138

1 x96 well plate

369 USD

Human Nesfatin-1 PicoKine ELISA Kit

EK1138

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Nesfatin-1

E.coli, V25-L106

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 146; Standard deviation: 7.6; CV(%): 5.2 Sample 2: n=16; Mean(pg/ml): 726; Standard deviation: 35.6; CV(%): 4.9 Sample 3: n=16; Mean(pg/ml): 1252; Standard deviation: 68.86; CV(%): 5.5

Sample 1: n=24; Mean(pg/ml): 177; Standard deviation: 9.56; CV(%): 5.4 Sample 2: n=24; Mean(pg/ml): 838; Standard deviation: 47.8; CV(%): 5.7 Sample 3: n=24; Mean(pg/ml): 1527; Standard deviation: 96.2; CV(%): 6.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Nesfatin-1 in cell culture supernates and serum.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Nesfatin-1 antibody 1 ST0000-10 Lyophilized recombinant human Nesfatin-1 standard 2ng/tubex2 AR1107 Biotinylated anti- human Nesfatin-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2(NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

NUCB2

Nucleobindin-2

nucleobindin 2

DNA binding protein NEFA DNA-binding protein NEFA Gastric cancer antigen Zg4 NEFA Nefastin 1 Nefastin-1 Nefastin1 Nucb2 NUCB2_HUMAN Nucleobindin-2 Nucleobinding 2 Prepronefastin Prepronesfatin

P80303