Boster Immunoleader

ELISA/assay

Mouse CCL12/MCP5 PicoKine ELISA Kit

EK1128

1 x96 well plate

369 USD

Mouse CCL12/MCP5 PicoKine ELISA Kit

EK1128

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse CCL12/MCP5

E.coli, G23-G104

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 113; Standard deviation: 4.86; CV(%): 4.3 Sample 2: n=16; Mean(pg/ml): 468; Standard deviation: 22.00; CV(%): 4.7 Sample 3: n=16; Mean(pg/ml): 1221; Standard deviation: 69.60; CV(%): 5.7

Sample 1: n=24; Mean(pg/ml): 126; Standard deviation: 10.46; CV(%): 8.3 Sample 2: n=24; Mean(pg/ml): 471; Standard deviation: 41.92; CV(%): 8.9 Sample 3: n=24; Mean(pg/ml): 1288; Standard deviation: 117.21; CV(%): 9.1

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse CCL12 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse CCL12 antibody 1 ST0000-10 Lyophilized recombinant mouse CCL12 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse CCL12 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Chemokine(C-C motif) ligand 12(CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5(MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.

Ccl12

C-C motif chemokine 12

chemokine (C-C motif) ligand 12

C C motif chemokine 12 Ccl12 MCP 1 related chemokine Monocyte chemoattractant protein 5 Monocyte chemotactic protein 5 Scya12 Small inducible cytokine A12

Q62401