Boster Immunoleader

ELISA/assay

Human A2M/alpha2-Macroglobulin PicoKine ELISA Kit

EK1118

1 x96 well plate

369 USD

Human A2M/alpha2-Macroglobulin PicoKine ELISA Kit

EK1118

369 USD

Sandwich ELISA kit for Quantitative Detection of Human A2M/alpha2-Macroglobulin

from human plasma

1 x96 well plate

625pg/ml-40,000pg/ml

<20pg/ml

Sample 1: n=16; Mean(ng/ml): 6.18; Standard deviation: 0.377; CV(%): 6.1 Sample 2: n=16; Mean(ng/ml): 12.04; Standard deviation: 0.771; CV(%): 6.4 Sample 3: n=16; Mean(ng/ml): 25.21; Standard deviation: 0.174; CV(%): 6.9

Sample 1: n=24; Mean(ng/ml): 6.72; Standard deviation: 0.457; CV(%): 6.8 Sample 2: n=24; Mean(ng/ml): 13.68; Standard deviation: 0.985; CV(%): 7.2 Sample 3: n=24; Mean(ng/ml): 26.81; Standard deviation: 2.12; CV(%): 7.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human A2M in cell culture supernates, serum and plasma(heparin, EDTA) .

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human A2M antibody 1 ST0000-10 Lyophilized human A2M standard 40ng/tubex2 AR1107 Biotinylated anti- human A2M antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

A2M

Alpha-2-macroglobulin

alpha-2-macroglobulin

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 A2m A2MD A2MG_HUMAN Alpha-2-M Alpha-2-macroglobulin C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 CPAMD5 FWP007 Pregnancy zone protein S863-7

P01023