Boster Immunoleader

ELISA/assay

Human Granzyme B PicoKine ELISA Kit

EK1114

1 x96 well plate

369 USD

Human Granzyme B PicoKine ELISA Kit

EK1114

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Granzyme B

NSO, I21-Y247

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 89.5; Standard deviation: 4.65; CV(%): 5.2 Sample 2: n=16; Mean(pg/ml): 376; Standard deviation: 16.17; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 525; Standard deviation: 23.10; CV(%): 4.4

Sample 1: n=24; Mean(pg/ml): 97.6; Standard deviation: 7.32; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 368; Standard deviation: 25.39; CV(%): 6.9 Sample 3: n=24; Mean(pg/ml): 567; Standard deviation: 44.23; CV(%): 7.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Granzyme B in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Granzyme B antibody 1 ST0000-10 Lyophilized recombinant human Granzyme B standard 10ng/tubex2 AR1107 Biotinylated anti- human Granzyme B antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes(CTL) and natural killer(NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

GZMB

Granzyme B

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

C11 Cathepsin G like 1 CTLA 1 CTSGL1 Cytotoxic T lymphocyte proteinase 2 EC 3.4.21.79 Fragmentin 2 Granzyme 2 HLP Human lymphocyte protein Lymphocyte protease SECT T cell serine protease 1 3E

P10144