Boster Immunoleader

ELISA/assay

Rat FAS-L PicoKine ELISA Kit

EK1110

1 x96 well plate

369 USD

Rat FAS-L PicoKine ELISA Kit

EK1110

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat FAS-L

NSO, Q127-L278

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 118; Standard deviation: 4.13; CV(%): 3.5 Sample 2: n=16; Mean(pg/ml): 854; Standard deviation: 40.99; CV(%): 4.8 Sample 3: n=16; Mean(pg/ml): 1319; Standard deviation: 71.23; CV(%): 5.4

Sample 1: n=24; Mean(pg/ml): 102; Standard deviation: 5.2; CV(%): 5.1 Sample 2: n=24; Mean(pg/ml): 847; Standard deviation: 52.51; CV(%): 6.2 Sample 3: n=24; Mean(pg/ml): 1423; Standard deviation: 103.9; CV(%): 7.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat FAS-L in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat FAS-L antibody 1 ST0000-10 Lyophilized recombinant rat FAS-L standard 10ng/tubex2 AR1107 Biotinylated anti- rat FAS-L antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

FASLG

Tumor necrosis factor ligand superfamily member 6

Fas ligand (TNF superfamily, member 6)

Apoptosis (APO 1) antigen ligand 1 Apoptosis antigen ligand APT1LG1 APTL CD178 CD95 L CD95 ligand CD95L CD95L protein Fas antigen ligand FASL FASLG TNF superfamily member 6 TNFSF6 Tumor necrosis factor (ligand) superfamily member 6 Tumor necrosis factor ligand superfamily member 6

P36940