Boster Immunoleader

ELISA/assay

Rat Cystatin C PicoKine ELISA Kit

EK1109

1 x96 well plate

369 USD

Rat Cystatin C PicoKine ELISA Kit

EK1109

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat Cystatin C

NSO, M1-A140

1 x96 well plate

312pg/ml-20,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 3.11; Standard deviation: 0.140; CV(%): 4.5 Sample 2: n=16; Mean(ng/ml): 6.32; Standard deviation: 0.202; CV(%): 3.2 Sample 3: n=16; Mean(ng/ml): 13.24; Standard deviation: 0.543; CV(%): 4.1

Sample 1: n=24; Mean(ng/ml): 3.19; Standard deviation: 0.230; CV(%): 7.2 Sample 2: n=24; Mean(ng/ml): 6.41; Standard deviation: 0.391; CV(%): 6.1 Sample 3: n=24; Mean(ng/ml): 13.57; Standard deviation: 0.909; CV(%): 6.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat Cystatin C in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat Cystatin C antibody 1 ST0000-20 Lyophilized recombinant rat Cystatin C standard 20ng/tubex2 AR1107 Biotinylated anti- rat Cystatin C antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Cystatin C or cystatin 3(formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid(CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight(approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus(cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases(enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases(it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

CST3

Cystatin-C

cystatin C

ARMD11 bA218C14.4 (cystatin C) Cst3 Cystatin-3 Cystatin-C CYTC_HUMAN Gamma-trace Neuroendocrine basic polypeptide Post-gamma-globulin

P14841