Boster Immunoleader

ELISA/assay

Human Seprase/FAP PicoKine ELISA Kit

EK1101

1 x96 well plate

399 USD

Human Seprase/FAP PicoKine ELISA Kit

EK1101

399 USD

Sandwich ELISA kit for Quantitative Detection of Human Seprase/FAP

NSO, L26-D760

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 522; Standard deviation: 18.27; CV(%): 3.5 Sample 2: n=16; Mean(pg/ml): 1007; Standard deviation: 43.3; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 2116; Standard deviation: 105.8; CV(%): 5

Sample 1: n=24; Mean(pg/ml): 558; Standard deviation: 26.23; CV(%): 4.7 Sample 2: n=24; Mean(pg/ml): 1143; Standard deviation: 67.44; CV(%): 5.9 Sample 3: n=24; Mean(pg/ml): 2236; Standard deviation: 141; CV(%): 6.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Seprase in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Seprase antibody 1 ST0000-10 Lyophilized recombinant human Seprase standard 10ng/tubex2 AR1107 Biotinylated anti- human Seprase antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

FAP

Seprase

fibroblast activation protein, alpha

170 kDa melanoma membrane-bound gelatinase FAP Fibroblast activation protein alpha Integral membrane serine protease SEPR_HUMAN Seprase

Q12884