Boster Immunoleader

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Enhanced Chemiluminescent Kit (Mouse IgM) for Western Blotting

EK1005

1 kit

100 USD

Enhanced Chemiluminescent Kit (Mouse IgM) for Western Blotting

EK1005

100 USD

1 kit

4˚C for 1 year.

WB

1. Blocking buffer: 10g protein dry powder (skimmed milk powder). 2. HRP‐conjugated goat anti‐mouse IgM: Affinity purified antibody. 2000‐10000x. 3. Enhanced chemiluminescent chromogenic reagent, containing: •Chromogenic reagent A: 5ml. 20x. •Chromogenic reagent B: 5ml. 20x.

•Nitrocellulose or PVDF membrane. •Diluent Buffer (for preparation of blocking reagent and antibody dilutions): Add 2.42g Tris, 9g NaCl, 850‐900ul pure acetic acid into 1000ml distilled water, adjust pH to 7.2‐7.6. •Wash Buffer: Add 0.5 ml of TWEEN 20 into 1000 ml of diluent buffer. •Primary antibody: User provided. This kit applies to the primary IgM antibodies raised from mouse.

• “Mouse IgM” refers to the origin of animal species of the primary antibody, not the origin of the specimen. This kit applies to the primary antibodies raised from mouse. • Every kit has sufficient reagents for 800 cm2 of membranes.