Boster Immunoleader

ELISA/assay

Human ALCAM PicoKine ELISA Kit

EK0995

1 x96 well plate

369 USD

Human ALCAM PicoKine ELISA Kit

EK0995

369 USD

Sandwich ELISA kit for Quantitative Detection of Human ALCAM

NSO, W28-A526

1 x96 well plate

312pg/ml-20,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(ng/ml): 3.07; Standard deviation: 0.150; CV(%): 4.9 Sample 2: n=16; Mean(ng/ml): 6.42; Standard deviation: 0.327; CV(%): 5.1 Sample 3: n=16; Mean(ng/ml): 13.26; Standard deviation: 0.756; CV(%): 5.7

Sample 1: n=24; Mean(ng/ml): 3.58; Standard deviation: 0.218; CV(%): 6.1 Sample 2: n=24; Mean(ng/ml): 7.04; Standard deviation: 0.444; CV(%): 6.3 Sample 3: n=24; Mean(ng/ml): 14.98; Standard deviation: 0.959; CV(%): 6.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human ALCAM in cell culture supernates, serum and plasma(heparin , EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human ALCAM antibody 1 ST0000-10 Lyophilized recombinant human ALCAM standard 20ng/tubex2 AR1107 Biotinylated anti- human ALCAM antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166(cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

ALCAM

CD166 antigen

activated leukocyte cell adhesion molecule

Activated leukocyte cell adhesion molecule ALCAM ALCAM protein CD 166 CD166 CD166 antigen CD166_HUMAN FLJ3851 FLJ38514 MEMD MGC71733

Q13740