Boster Immunoleader

ELISA/assay

Mouse Complement C5a PicoKine ELISA Kit

EK0987

1 x96 well plate

369 USD

Mouse Complement C5a PicoKine ELISA Kit

EK0987

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Complement C5a

E.coli, N679-R755

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 37.7; Standard deviation: 1.39; CV(%): 3.7 Sample 2: n=16; Mean(pg/ml): 118; Standard deviation: 5.43; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 621; Standard deviation: 32.91; CV(%): 5.3

Sample 1: n=24; Mean(pg/ml): ; Standard deviation: ; CV(%): Sample 2: n=24; Mean(pg/ml): ; Standard deviation: ; CV(%): Sample 3: n=24; Mean(pg/ml): ; Standard deviation: ; CV(%):

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse Complement C5a in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse Complement C5a antibody 1 ST0000-10 Lyophilized recombinant mouse Complement C5a standard 10ng/tubex2 AR1107 Biotinylated anti- mouse Complement C5a antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

C5

Complement C5

complement component 5

anaphylatoxin C5a analog C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 C5 CO5_HUMAN complement C5 Complement C5 alpha chain Complement component 5 CPAMD4 ECLZB

P06684