Boster Immunoleader

ELISA/assay

Human Endothelin PicoKine ELISA Kit

EK0945

1 x96 well plate

369 USD

Human Endothelin PicoKine ELISA Kit

EK0945

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Endothelin

human peptide, C53-W73

1 x96 well plate

3.9pg/ml-250pg/ml

<0.5pg/ml

Sample 1: n=16; Mean(pg/ml): 25.1; Standard deviation: 1.41; CV(%): 5.6 Sample 2: n=16; Mean(pg/ml): 85.4; Standard deviation: 3.76; CV(%): 4.4 Sample 3: n=16; Mean(pg/ml): 146; Standard deviation: 8.03; CV(%): 5.5

Sample 1: n=24; Mean(pg/ml): 23.2; Standard deviation: 1.79; CV(%): 7.7 Sample 2: n=24; Mean(pg/ml): 84.3; Standard deviation: 6.07; CV(%): 7.2 Sample 3: n=24; Mean(pg/ml): 143; Standard deviation: 8.72; CV(%): 6.1

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Endothelin in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Endothelin antibody 1 ST0000-10 Lyophilized recombinant human Endothelin standard 1ng/tubex2 AR1107 Biotinylated anti- human Endothelin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

EDN1

Endothelin-1

endothelin 1

Big endothelin 1 ET1 HDLCQ7 PPET1 Preproendothelin 1 EDN2 EDN2_HUMAN Endothelin 2 Endothelin-2 ET 2 ET-2 ET2 PPET2 Preproendothelin 2 Preproendothelin-2 EDN3 EDN3_HUMAN Endothelin-3 ET 3 ET-3 PPET3 Preproendothelin 3 Preproendothelin-3

P05305