Boster Immunoleader

ELISA/assay

Human MIA PicoKine ELISA Kit

EK0941

1 x96 well plate

369 USD

info changed 20150527

Human MIA PicoKine ELISA Kit

EK0941

369 USD

Sandwich ELISA kit for Quantitative Detection of Human MIA

E.coli, G25-131Q

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 139; Standard deviation: 5.42; CV(%): 3.9 Sample 2: n=16; Mean(pg/ml): 325; Standard deviation: 16.25; CV(%): 5 Sample 3: n=16; Mean(pg/ml): 642; Standard deviation: 36.6; CV(%): 5.7

Sample 1: n=24; Mean(pg/ml): 157; Standard deviation: 7.54; CV(%): 4.8 Sample 2: n=24; Mean(pg/ml): 368; Standard deviation: 22.45; CV(%): 6.1 Sample 3: n=24; Mean(pg/ml): 667; Standard deviation: 46; CV(%): 6.9

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MIA in cell culture supernates, serum, plasma(heparin, EDTA) and saliva.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MIA antibody 1 ST0000-10 Lyophilized recombinant human MIA standard 1ng/tubex2 AR1107 Biotinylated anti- human MIA antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

MIA

Melanoma-derived growth regulatory protein

melanoma inhibitory activity

CD RAP melanoma inhibitory activity Melanoma inhibitory activity protein Melanoma-derived growth regulatory protein Mia MIA_HUMAN

Q16674