Boster Immunoleader

ELISA/assay

Rat CCL4/MIP-1 beta PicoKine ELISA Kit

EK0939

1 x96 well plate

369 USD

Rat CCL4/MIP-1 beta PicoKine ELISA Kit

EK0939

369 USD

Sandwich ELISA kit for Quantitative Detection of Rat CCL4/MIP-1 beta

E.Coli, A24-N92

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 35; Standard deviation: 1.3; CV(%): 3.7 Sample 2: n=16; Mean(pg/ml): 112; Standard deviation: 6.1; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 367; Standard deviation: 21.3; CV(%): 5.8

Sample 1: n=24; Mean(pg/ml): 36; Standard deviation: 1.9; CV(%): 5.3 Sample 2: n=24; Mean(pg/ml): 116; Standard deviation: 7.4; CV(%): 6.4 Sample 3: n=24; Mean(pg/ml): 371; Standard deviation: 26.7; CV(%): 7.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat CCL4 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat CCL4/MIP-1 beta antibody 1 ST0000-10 Lyophilized recombinant rat CCL4/MIP-1 beta standard 10ng/tubex2 AR1107 Biotinylated anti- rat CCL4/MIP-1 beta antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14).

CCL4

C-C motif chemokine 4

chemokine (C-C motif) ligand 4

ACT-2 ACT2 AT744.1 C-C motif chemokine 4 CC chemokine ligand 4 CCL4 CCL4_HUMAN G-26 T-lymphocyte-secreted protein HC21 LAG-1 LAG1 Lymphocyte activation gene 1 protein Macrophage inflammatory protein 1-beta MIP-1-beta MIP-1-beta(1-69) MIP-1-beta(3-69) MIP1-B MIP1B MIP1B1 PAT 744 Protein H400 SCYA2 SCYA4 SIS-gamma Small-inducible cytokine A4 T-cell activation protein 2

P50230