Boster Immunoleader

ELISA/assay

Mouse IL-12(p40) PicoKine ELISA Kit

EK0932

1 x96 well plate

333 USD

Mouse IL-12(p40) PicoKine ELISA Kit

EK0932

333 USD

Sandwich ELISA kit for Quantitative Detection of Mouse IL-12(p40)

sf21, M23-S335

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 136; Standard deviation: 8.3; CV(%): 6.1 Sample 2: n=16; Mean(pg/ml): 390; Standard deviation: 20.28; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 683; Standard deviation: 31.42; CV(%): 4.6

Sample 1: n=24; Mean(pg/ml): 165; Standard deviation: 11.55; CV(%): 7 Sample 2: n=24; Mean(pg/ml): 446; Standard deviation: 30.33; CV(%): 6.8 Sample 3: n=24; Mean(pg/ml): 716; Standard deviation: 40.1; CV(%): 5.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse IL-12(p40) in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse IL-12(p40) antibody 1 ST0000-10 Lyophilized recombinant mouse IL-12(p40) standard 10ng/tubex2 AR1107 Biotinylated anti- mouse IL-12(p40) antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit(p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

IL12B

Interleukin-12 subunit beta

interleukin 12B

CLMF CLMF p40 CLMF2 Cytotoxic lymphocyte maturation factor 40 kDa subunit IL 12B IL-12 subunit p40 IL-12B IL12 p40 IL12B IL12B_HUMAN Interleukin 12 subunit beta NK cell stimulatory factor chain 2 NKSF NKSF2

P43432