Boster Immunoleader

ELISA/assay

Human IL-33 PicoKine ELISA Kit

EK0929

1 x96 well plate

333 USD

Human IL-33 PicoKine ELISA Kit

EK0929

333 USD

Sandwich ELISA kit for Quantitative Detection of Human IL-33

E.coli, S112-T270

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 74; Standard deviation: 3.48; CV(%): 4.7 Sample 2: n=16; Mean(pg/ml): 383; Standard deviation: 19.15; CV(%): 5 Sample 3: n=16; Mean(pg/ml): 644; Standard deviation: 39.3; CV(%): 6.1

Sample 1: n=24; Mean(pg/ml): 91; Standard deviation: 5.2; CV(%): 5.7 Sample 2: n=24; Mean(pg/ml): 411; Standard deviation: 22.61; CV(%): 5.5 Sample 3: n=24; Mean(pg/ml): 738; Standard deviation: 49.45; CV(%): 6.7

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human IL-33 in cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human IL-33 antibody 1 ST0000-10 Lyophilized recombinant human IL-33 standard 10ng/tubex2 AR1107 Biotinylated anti- human IL-33 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

IL33

Interleukin-33

interleukin 33

C9orf26 DVS27 DVS27 related protein IL-1F11 IL-33 IL1F11 IL33 IL33_HUMAN Interleukin-1 family member 11 Interleukin-33 NF-HEV NFEHEV Nuclear factor from high endothelial venules RP11-575C20.2

O95760