Boster Immunoleader

ELISA/assay

Human Thrombomodulin PicoKine ELISA Kit

EK0917

1 x96 well plate

369 USD

Human Thrombomodulin PicoKine ELISA Kit

EK0917

369 USD

Sandwich ELISA kit for Quantitative Detection of Human Thrombomodulin

NSO, A19-S515

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 523; Standard deviation: 24.1; CV(%): 4.6 Sample 2: n=16; Mean(pg/ml): 1074; Standard deviation: 40.1; CV(%): 3.7 Sample 3: n=16; Mean(pg/ml): 2246; Standard deviation: 92.1; CV(%): 4.1

Sample 1: n=24; Mean(pg/ml): 576; Standard deviation: 47.2; CV(%): 8.2 Sample 2: n=24; Mean(pg/ml): 1187; Standard deviation: 86.7; CV(%): 7.3 Sample 3: n=24; Mean(pg/ml): 2315; Standard deviation: 180.6; CV(%): 7.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human Thrombomodulin in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human Thrombomodulin antibody 1 ST0000-10 Lyophilized recombinant human Thrombomodulin standard 10ng/tubex2 AR1107 Biotinylated anti- human Thrombomodulin antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).

THBD

Thrombomodulin

thrombomodulin

CD141 CD141 antigen Fetomodulin Thbd THRM Thrombomodulin TM TRBM_HUMAN

P07204