Boster Immunoleader

ELISA/assay

Rat MCP-1/CCL2 PicoKine ELISA Kit

EK0902

1 x96 well plate

333 USD

Rat MCP-1/CCL2 PicoKine ELISA Kit

EK0902

333 USD

Sandwich ELISA kit for Quantitative Detection of Rat MCP-1/CCL2

NSO, Q24-N148

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 87; Standard deviation: 3.306; CV(%): 3.8 Sample 2: n=16; Mean(pg/ml): 318; Standard deviation: 16.54; CV(%): 5.2 Sample 3: n=16; Mean(pg/ml): 627; Standard deviation: 36.4; CV(%): 5.8

Sample 1: n=24; Mean(pg/ml): 98; Standard deviation: 4.802; CV(%): 4.9 Sample 2: n=24; Mean(pg/ml): 346; Standard deviation: 18.34; CV(%): 5.3 Sample 3: n=24; Mean(pg/ml): 683; Standard deviation: 42.35; CV(%): 6.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat MCP-1 in cell culture supernates and serum.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat MCP-1/CCL2 antibody 1 ST0000-10 Lyophilized recombinant rat MCP-1/CCL2 standard 10ng/tubex2 AR1107 Biotinylated anti- rat MCP-1/CCL2 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

CCL2

C-C motif chemokine 2

chemokine (C-C motif) ligand 2

C-C motif chemokine 2 CCL2 CCL2_HUMAN Chemokine (C-C motif) ligand 2 GDCF-2 HC11 HSMCR30 MCAF MCP-1 MCP1 MGC9434 Monocyte chemoattractant protein 1 Monocyte chemoattractant protein-1 Monocyte chemotactic and activating factor Monocyte chemotactic protein 1 Monocyte secretory protein JE SCYA2 Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je) Small inducible cytokine subfamily A (Cys-Cys), member 2 Small-inducible cytokine A2 SMC-CF

P14844