Boster Immunoleader

ELISA/assay

Human PEDF/SerpinF1 PicoKine ELISA Kit

EK0896

1 x96 well plate

369 USD

Human PEDF/SerpinF1 PicoKine ELISA Kit

EK0896

369 USD

Sandwich ELISA kit for Quantitative Detection of Human PEDF/SerpinF1

NSO, M1-P418

1 x96 well plate

0.78ng/ml-50ng/ml

<20pg/ml

Sample 1: n=16; Mean(ng/ml): 5.62; Standard deviation: 0.259; CV(%): 4.6 Sample 2: n=16; Mean(ng/ml): 10.1; Standard deviation: 0.444; CV(%): 4.4 Sample 3: n=16; Mean(ng/ml): 25.5; Standard deviation: 1.07; CV(%): 4.2

Sample 1: n=24; Mean(ng/ml): 6.23; Standard deviation: 0.467; CV(%): 7.5 Sample 2: n=24; Mean(ng/ml): 12.1; Standard deviation: 0.835; CV(%): 6.9 Sample 3: n=24; Mean(ng/ml): 26.4; Standard deviation: 1.95; CV(%): 7.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human PEDF in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human PEDF antibody 1 ST0000-50 Lyophilized recombinant human PEDF standard 50ng/tube×2 AR1107 Biotinylated anti- human PEDF antibody 130μl(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

SERPINF1

Pigment epithelium-derived factor

serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1

Alpha 2 antiplasmin Cell proliferation-inducing gene 35 protein EPC 1 EPC-1 EPC1 OI12 OI6 PEDF PEDF_HUMAN PIG 35 PIG35 Pigment epithelium derived factor Pigment epithelium-derived factor Proliferation inducing protein 35 serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 SERPIN F1 Serpin peptidase inhibitor clade F member 1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 SERPINF 1 SERPINF1

P36955