Boster Immunoleader

ELISA/assay

Human HSP27 PicoKine ELISA Kit

EK0881

1 x96 well plate

380 USD

Human HSP27 PicoKine ELISA Kit

EK0881

380 USD

Sandwich ELISA kit for Quantitative Detection of Human HSP27

E.coli, T2-K205

1 x96 well plate

62.5pg/ml-4000pg/ml

<5pg/ml

Sample 1: n=16; Mean(pg/ml): 256; Standard deviation: 10.75; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 1369; Standard deviation: 73.9; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 2628; Standard deviation: 144.5; CV(%): 5.5

Sample 1: n=24; Mean(pg/ml): 279; Standard deviation: 17.6; CV(%): 6.3 Sample 2: n=24; Mean(pg/ml): 1406; Standard deviation: 105.45; CV(%): 7.5 Sample 3: n=24; Mean(pg/ml): 2635; Standard deviation: 213.4; CV(%): 8.1

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human HSP27 in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and tissue homogenates.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human HSP27 antibody 1 ST0000-10 Lyophilized recombinant human HSP27 standard 10ng/tubex2 AR1107 Biotinylated anti- human HSP27 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.

HSPB1

Heat shock protein beta-1

heat shock 27kDa protein 1

HSPB1 28 kDa heat shock protein CMT2F estrogen regulated 24 kDa protein Estrogen-regulated 24 kDa protein Heat shock 27 kDa protein heat shock 27kDa protein 1 heat shock protein beta 1 Heat shock protein beta-1 HMN2B HS.76067 HSP 27 Hsp25 HSP27 HSP28 HspB1 SRP27 stress responsive protein 27 Stress-responsive protein 27

P04792