Boster Immunoleader

ELISA/assay

Mouse KIM1 PicoKine ELISA Kit

EK0880

1 x96 well plate

380 USD

Mouse KIM1 PicoKine ELISA Kit

EK0880

380 USD

Sandwich ELISA kit for Quantitative Detection of Mouse KIM1

NSO, Y22-T212

1 x96 well plate

31.2pg/ml-2000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 306; Standard deviation: 12.24; CV(%): 4 Sample 2: n=16; Mean(pg/ml): 640; Standard deviation: 35.2; CV(%): 5.5 Sample 3: n=16; Mean(pg/ml): 1118; Standard deviation: 69.32; CV(%): 6.2

Sample 1: n=24; Mean(pg/ml): 418; Standard deviation: 24.7; CV(%): 5.9 Sample 2: n=24; Mean(pg/ml): 825; Standard deviation: 58.6; CV(%): 7.1 Sample 3: n=24; Mean(pg/ml): 1263; Standard deviation: 96; CV(%): 7.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of Mouse KIM1 in cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) and urine.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse KIM1 antibody 1 ST0000-10 Lyophilized recombinant mouse KIM1 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse KIM1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al.(1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al.(2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al.(2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

HAVCR1

Hepatitis A virus cellular receptor 1

hepatitis A virus cellular receptor 1

HAVCR HAVcr-1 Havcr1 HAVR1_HUMAN Hepatitis A virus cellular receptor 1 Kidney injury molecule 1 KIM-1 T cell immunoglobin domain and mucin domain protein 1 T-cell immunoglobulin and mucin domain-containing protein 1 T-cell membrane protein 1 TIM TIM-1 TIM1 TIMD-1 TIMD1

Q3V033