Boster Immunoleader

ELISA/assay

Mouse PECAM-1/CD31 PicoKine ELISA Kit

EK0874

1 x96 well plate

420 USD

Mouse PECAM-1/CD31 PicoKine ELISA Kit

EK0874

420 USD

Sandwich ELISA kit for Quantitative Detection of Mouse PECAM-1/CD31

NSO, E18-K590

1 x96 well plate

156pg/ml-10000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1425; Standard deviation: 89.8; CV(%): 6.3 Sample 2: n=16; Mean(pg/ml): 2256; Standard deviation: 169.2; CV(%): 7.5 Sample 3: n=16; Mean(pg/ml): 4926; Standard deviation: 280.8; CV(%): 5.7

Sample 1: n=24; Mean(pg/ml): 1614; Standard deviation: 111.4; CV(%): 6.9 Sample 2: n=24; Mean(pg/ml): 2648; Standard deviation: 214.5; CV(%): 8.1 Sample 3: n=24; Mean(pg/ml): 5830; Standard deviation: 378.95; CV(%): 6.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse PECAM-1 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse PECAM-1/CD31 antibody 1 ST0000-10 Lyophilized recombinant mouse PECAM-1/CD31 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse PECAM-1/CD31 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.

PECAM1

Platelet endothelial cell adhesion molecule

platelet/endothelial cell adhesion molecule 1f

CD31 CD31 antigen EndoCAM GPIIA HEC7 Leukocyte surface protein PECA1 PECA1_HUMAN PECAM 1 PECAM-1 Pecam1 Platelet endothelial cell adhesion molecule Platelet/endothelial cell adhesion molecule

Q08481