Boster Immunoleader

ELISA/assay

Human PECAM-1/CD31 PicoKine ELISA Kit

EK0873

1 x96 well plate

420 USD

Human PECAM-1/CD31 PicoKine ELISA Kit

EK0873

420 USD

Sandwich ELISA kit for Quantitative Detection of Human PECAM-1/CD31

CHO, Q28-K601

1 x96 well plate

156pg/ml-10000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1282; Standard deviation: 87.2; CV(%): 6.8 Sample 2: n=16; Mean(pg/ml): 2520; Standard deviation: 120.96; CV(%): 4.8 Sample 3: n=16; Mean(pg/ml): 5023; Standard deviation: 261.2; CV(%): 5.2

Sample 1: n=24; Mean(pg/ml): 1526; Standard deviation: 116; CV(%): 7.6 Sample 2: n=24; Mean(pg/ml): 2768; Standard deviation: 182.7; CV(%): 6.6 Sample 3: n=24; Mean(pg/ml): 5281; Standard deviation: 338; CV(%): 6.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human PECAM-1 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human PECAM-1/CD31 antibody 1 ST0000-10 Lyophilized recombinant human PECAM-1/CD31 standard 10ng/tubex2 AR1107 Biotinylated anti- human PECAM-1/CD31 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Human platelet/endothelial cell adhesion molecule-1(PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 574 amino acids(Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.

PECAM1

Platelet endothelial cell adhesion molecule

platelet/endothelial cell adhesion molecule 1f

CD31 CD31 antigen EndoCAM GPIIA HEC7 Leukocyte surface protein PECA1 PECA1_HUMAN PECAM 1 PECAM-1 Pecam1 Platelet endothelial cell adhesion molecule Platelet/endothelial cell adhesion molecule

P16284