Boster Immunoleader

ELISA/assay

Human PAI-1 PicoKine ELISA Kit

EK0859

1 x96 well plate

400 USD

Human PAI-1 PicoKine ELISA Kit

EK0859

400 USD

Sandwich ELISA kit for Quantitative Detection of Human PAI-1

sf21, S22-P402

1 x96 well plate

156pg/ml-10,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1539; Standard deviation: 76.95; CV(%): 5 Sample 2: n=16; Mean(pg/ml): 3736; Standard deviation: 235.4; CV(%): 6.3 Sample 3: n=16; Mean(pg/ml): 6234; Standard deviation: 405.21; CV(%): 6.5

Sample 1: n=24; Mean(pg/ml): 1637; Standard deviation: 104.8; CV(%): 6.4 Sample 2: n=24; Mean(pg/ml): 3914; Standard deviation: 293.55; CV(%): 7.5 Sample 3: n=24; Mean(pg/ml): 7335; Standard deviation: 601.47; CV(%): 8.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human PAI-1 in cell culture supernates, cell lysates, serum and plasma(heparin, EDTA, citrate ).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human PAI-1 antibody 1 ST0000-10 Lyophilized recombinant human PAI-1 standard 10ng/tubex2 AR1107 Biotinylated anti- human PAI-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator(tPA) and urokinase(uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine protease inhibitor(serpin) protein(SERPINE1). PAI-1 is mainly produced by the endothelium(cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome(7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis(a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states(such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

SERPINE1

Plasminogen activator inhibitor 1

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Endothelial plasminogen activator inhibitor PAI PAI-1 PAI1 PAI1_HUMAN PLANH1 Plasminogen activator inhibitor 1 Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serpin E1 Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 SERPINE1

P05121