Boster Immunoleader

ELISA/assay

Mouse TNFSF4/OX40L PicoKine ELISA Kit

EK0858

1 x96 well plate

333 USD

Mouse TNFSF4/OX40L PicoKine ELISA Kit

EK0858

333 USD

Sandwich ELISA kit for Quantitative Detection of Mouse TNFSF4/OX40L

NSO, S51-L198

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 534; Standard deviation: 23.5; CV(%): 4.4 Sample 2: n=16; Mean(pg/ml): 1107; Standard deviation: 43.2; CV(%): 3.9 Sample 3: n=16; Mean(pg/ml): 2374; Standard deviation: 125.8; CV(%): 5.3

Sample 1: n=24; Mean(pg/ml): 639; Standard deviation: 37.1; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 1320; Standard deviation: 60.7; CV(%): 4.6 Sample 3: n=24; Mean(pg/ml): 2448; Standard deviation: 156.7; CV(%): 6.4

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse TNFSF4 in cell culture supernates, serum and plasma(heparin, EDTA).

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse TNFSF4antibody 1 ST0000-10 Lyophilized recombinant mouse TNFSF4 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse TNFSF4 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al.(1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.

TNFSF4

Tumor necrosis factor ligand superfamily member 4

tumor necrosis factor (ligand) superfamily, member 4

CD 134L CD 252 CD134 CD134 ligand CD134L CD252 CD252 antigen glycoprotein 34 kd Glycoprotein Gp34 GP34 OX-40L OX40 antigen ligand OX40 ligand OX40L TAX transcriptionally-activated glycoprotein 1 TNFL4_HUMAN Tnfsf4 Tumor necrosis factor (ligand) superfamily member 4 Tumor necrosis factor ligand superfamily member 4 TXGP1

P43488