Boster Immunoleader

ELISA/assay

Mouse Lipocalin-2/NGAL PicoKine ELISA Kit

EK0854

1 x96 well plate

400 USD

Mouse Lipocalin-2/NGAL PicoKine ELISA Kit

EK0854

400 USD

Sandwich ELISA kit for Quantitative Detection of Mouse Lipocalin-2/NGAL

NSO, Q21-N200

1 x96 well plate

78pg/ml-5,000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 527; Standard deviation: 35.84; CV(%): 6.8 Sample 2: n=16; Mean(pg/ml): 1729; Standard deviation: 77.81; CV(%): 4.5 Sample 3: n=16; Mean(pg/ml): 3028; Standard deviation: 148.4; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 722; Standard deviation: 53.43; CV(%): 7.4 Sample 2: n=24; Mean(pg/ml): 2011; Standard deviation: 104.6; CV(%): 5.2 Sample 3: n=24; Mean(pg/ml): 3316; Standard deviation: 199; CV(%): 6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse NGAL in cell culture supernates, serum, plasma(heparin) and urine.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse Lipocalin-2/NGAL antibody 1 ST0000-10 Lyophilized recombinant mouse Lipocalin-2/NGAL standard 10ng/tubex2 AR1107 Biotinylated anti- mouse Lipocalin-2/NGAL antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.

LCN2

Neutrophil gelatinase-associated lipocalin

lipocalin 2

24p3 25 kDa alpha-2-microglobulin-related subunit of MMP-9 HNL Lcn2 Lipocalin 2 Lipocalin-2 Migration stimulating factor inhibitor MSFI Neutrophil gelatinase-associated lipocalin NGAL NGAL_HUMAN Oncogene 24p3 p25 Siderocalin Siderocalin LCN2

P11672