Boster Immunoleader

ELISA/assay

Human CD10/Neprilysin PicoKine ELISA Kit

EK0851

1 x96 well plate

369 USD

Human CD10/Neprilysin PicoKine ELISA Kit

EK0851

369 USD

Sandwich ELISA kit for Quantitative Detection of Human CD10/Neprilysin

CHO, Y52-W750

1 x96 well plate

125pg/ml-8000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 1265; Standard deviation: 48.07; CV(%): 3.8 Sample 2: n=16; Mean(pg/ml): 3210; Standard deviation: 179.8; CV(%): 5.6 Sample 3: n=16; Mean(pg/ml): 6840; Standard deviation: 321.5; CV(%): 4.7

Sample 1: n=24; Mean(pg/ml): 1526; Standard deviation: 83.93; CV(%): 5.5 Sample 2: n=24; Mean(pg/ml): 2761; Standard deviation: 201; CV(%): 7.3 Sample 3: n=24; Mean(pg/ml): 6397; Standard deviation: 369.6; CV(%): 6.2

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human CD10 in cell culture supernates, serum and plasma(heparin, EDTA).

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human CD10 antibody 1 ST0000-10 Lyophilized recombinant human CD10 standard 10ng/tubex2 AR1107 Biotinylated anti- human CD10 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

MME

Neprilysin

membrane metallo-endopeptidase

Atriopeptidase CALLA CD 10 CD10 CD10 antigen Common acute lymphocytic leukemia antigen DKFZp686O16152 EC 3.4.24.11 Enkephalinase EPN gp100 Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) Membrane metallo endopeptidase Membrane metallo endopeptidase variant 1 Membrane metallo endopeptidase variant 2 Membrane metalloendopeptidase Membrane metalloendopeptidase neutral endopeptidase enkephalinase Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 Membrane metalloendopeptidase variant 1 Membrane metalloendopeptidase variant 2 MGC126681 MGC126707 MME NEP NEP_HUMAN Neprilysin Neutral endopeptidase 24.11 Neutral endopeptidase SFE Skin fibroblast elastase

P08473