Boster Immunoleader

ELISA/assay

Rat TrkA PicoKine ELISA Kit

EK0847

1 x96 well plate

420 USD

Rat TrkA PicoKine ELISA Kit

EK0847

420 USD

Sandwich ELISA kit for Quantitative Detection of Rat trkA

NSO, A33-P418

1 x96 well plate

156pg/ml-10000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 595; Standard deviation: 32.13; CV(%): 5.4 Sample 2: n=16; Mean(pg/ml): 3622; Standard deviation: 166.6; CV(%): 4.6 Sample 3: n=16; Mean(pg/ml): 6653; Standard deviation: 452.4; CV(%): 6.8

Sample 1: n=24; Mean(pg/ml): 614; Standard deviation: 38.68; CV(%): 6.3 Sample 2: n=24; Mean(pg/ml): 3125; Standard deviation: 162.5; CV(%): 5.2 Sample 3: n=24; Mean(pg/ml): 7234; Standard deviation: 542.6; CV(%): 7.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of rat TRKA in cell culture supernates, cell lysates and tissue homogenates.

Rat

Catalog number Description Quantity - 96-well plate precoated with anti- rat TrkA antibody 1 ST0000-10 Lyophilized recombinant rat TrkA standard 10ng/tubex2 AR1107 Biotinylated anti- rat TrkA antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.

NTRK1

High affinity nerve growth factor receptor

neurotrophic tyrosine kinase, receptor, type 1

High affinity nerve growth factor receptor MTC Neurotrophic tyrosine kinase receptor type 1 NTRK1 NTRK1_HUMAN Oncogene TRK p140-TrkA TRK Trk-A TRK1 TRK1-transforming tyrosine kinase protein

P35739