Boster Immunoleader

ELISA/assay

Mouse TREM-1 PicoKine ELISA Kit

EK0845

1 x96 well plate

369 USD

Mouse TREM-1 PicoKine ELISA Kit

EK0845

369 USD

Sandwich ELISA kit for Quantitative Detection of Mouse TREM-1

NSO, A21-S202

1 x96 well plate

31.2pg/ml-2000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 252; Standard deviation: 14.93; CV(%): 5.9 Sample 2: n=16; Mean(pg/ml): 559; Standard deviation: 39.13; CV(%): 7 Sample 3: n=16; Mean(pg/ml): 924; Standard deviation: 42.5; CV(%): 4.6

Sample 1: n=24; Mean(pg/ml): 314; Standard deviation: 24.5; CV(%): 7.8 Sample 2: n=24; Mean(pg/ml): 675; Standard deviation: 48.6; CV(%): 7.2 Sample 3: n=24; Mean(pg/ml): 1104; Standard deviation: 60.72; CV(%): 5.5

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of mouse TREM-1 in cell culture supernates, serum and plasma(heparin, EDTA), cell lysates and tissue homogenates.

Mouse

Catalog number Description Quantity - 96-well plate precoated with anti- mouse TREM-1 antibody 1 ST0000-10 Lyophilized recombinant mouse TREM-1 standard 10ng/tubex2 AR1107 Biotinylated anti- mouse TREM-1 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). Trem1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.

TREM1

Triggering receptor expressed on myeloid cells 1

triggering receptor expressed on myeloid cells 1

CD354 TREM 1Triggering receptor expressed on monocytes 1 Triggering receptor expressed on myeloid cells 1 Triggering receptor TREM 1 Triggering receptor TREM1

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