Boster Immunoleader

ELISA/assay

Human RANK PicoKine ELISA Kit

EK0829

1 x96 well plate

380 USD

Human RANK PicoKine ELISA Kit

EK0829

380 USD

Sandwich ELISA kit for Quantitative Detection of Human RANK

NSO, Q29-G213

1 x96 well plate

62.5pg/ml-4000pg/ml

<2pg/ml

Sample 1: n=16; Mean(pg/ml): 655; Standard deviation: 26.2; CV(%): 4 Sample 2: n=16; Mean(pg/ml): 1863; Standard deviation: 68.93; CV(%): 3.7 Sample 3: n=16; Mean(pg/ml): 2533; Standard deviation: 136.8; CV(%): 5.4

Sample 1: n=24; Mean(pg/ml): 639; Standard deviation: 37.1; CV(%): 5.8 Sample 2: n=24; Mean(pg/ml): 1957; Standard deviation: 90; CV(%): 4.6 Sample 3: n=24; Mean(pg/ml): 2668; Standard deviation: 176.1; CV(%): 6.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human RANK in cell culture supernates and serum. Plasma should be validated by end users.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human RANK antibody 1 ST0000-10 Lyophilized recombinant human RANK standard 10ng/tubex2 AR1107 Biotinylated anti- human RANK antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

TNFRSF11A

Tumor necrosis factor receptor superfamily member 11A

tumor necrosis factor receptor superfamily, member 11a, NFKB activator

CD265 FEO LOH18CR1 Loss of heterozygosity 18 chromosomal region 1 ODFR OFE OPTB7 Osteoclast differentiation factor receptor OSTS PDB2 Receptor activator of NF-KB Receptor activator of nuclear factor kappa B TNFRSF11A TNR11_HUMAN TRANCER Tumor necrosis factor receptor superfamily member 11A Tumor necrosis factor receptor superfamily member 11a activator of NFKB Tumor necrosis factor receptor superfamily member 11a NFKB activator

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