Boster Immunoleader

ELISA/assay

Human MIF PicoKine ELISA Kit

EK0813

1 x96 well plate

400 USD

Human MIF PicoKine ELISA Kit

EK0813

400 USD

Sandwich ELISA kit for Quantitative Detection of Human MIF

E.coli, P2-A115

1 x96 well plate

156pg/ml-10,000pg/ml

<20pg/ml

Sample 1: n=16; Mean(pg/ml): 1442; Standard deviation: 90.85; CV(%): 6.3 Sample 2: n=16; Mean(pg/ml): 3826; Standard deviation: 206.6; CV(%): 5.4 Sample 3: n=16; Mean(pg/ml): 6427; Standard deviation: 315; CV(%): 4.9

Sample 1: n=24; Mean(pg/ml): 1625; Standard deviation: 121.9; CV(%): 7.5 Sample 2: n=24; Mean(pg/ml): 4177; Standard deviation: 254.8; CV(%): 6.1 Sample 3: n=24; Mean(pg/ml): 6782; Standard deviation: 393.4; CV(%): 5.8

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MIF in cell culture supernates, serum, plasma(heparin, EDTA) and human milk.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MIF antibody 1 ST0000-10 Lyophilized recombinant human MIF standard 10ng/tubex2 AR1107 Biotinylated anti- human MIF antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

Macrophage migration inhibitory factor(MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4. The standard product used in this kit is recombinant human MIF, consisting of 115 amino acids with the molecular mass of 12.5kDa.

MIF

Macrophage migration inhibitory factor

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

GIF GLIF Glycosylation inhibiting factor L dopachrome isomerase L dopachrome tautomerase Macrophage migration inhibitory factor Macrophage migration inhibitory factor (glycosylation inhibiting factor) MMIF Phenylpyruvate tautomerase

I4AY87