Boster Immunoleader

ELISA/assay

Human MICA PicoKine ELISA Kit

EK0812

1 x96 well plate

400 USD

Human MICA PicoKine ELISA Kit

EK0812

400 USD

Sandwich ELISA kit for Quantitative Detection of Human MICA

NSO, E24-W307

1 x96 well plate

62.5pg/ml-4000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 447; Standard deviation: 22.8; CV(%): 5.1 Sample 2: n=16; Mean(pg/ml): 1327; Standard deviation: 75.64; CV(%): 5.7 Sample 3: n=16; Mean(pg/ml): 2443; Standard deviation: 151.5; CV(%): 6.2

Sample 1: n=24; Mean(pg/ml): 526; Standard deviation: 34.2; CV(%): 6.5 Sample 2: n=24; Mean(pg/ml): 1648; Standard deviation: 115.4; CV(%): 7 Sample 3: n=24; Mean(pg/ml): 2756; Standard deviation: 209.5; CV(%): 7.6

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human MICA in cell culture supernates, cell lysates, tissue homogenates and serum.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human MICA antibody 1 ST0000-10 Lyophilized recombinant human MICA standard 10ng/tubex2 AR1107 Biotinylated anti- human MICA antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes. The standard product used in this kit is recombinant MICA, which is composed of 286 amino acids with the molecular mass of 59KDa.

MICA

MHC class I polypeptide-related sequence A

MHC class I polypeptide-related sequence A

FLJ60820 HLA class I antigen MGC111087 MHC class I chain related gene A protein MHC class I chain related protein A MHC class I polypeptide related sequence A MHC class I related protein MIC A PERB11.1 Stress inducible class I homolog

Q29983