Boster Immunoleader

ELISA/assay

Human ErbB-2 PicoKine ELISA Kit

EK0756

1 x96 well plate

400 USD

Human ErbB-2 PicoKine ELISA Kit

EK0756

400 USD

Sandwich ELISA kit for Quantitative Detection of Human ErbB-2

NSO, T23-T652

1 x96 well plate

15.6pg/ml-1000pg/ml

<10pg/ml

Sample 1: n=16; Mean(pg/ml): 348; Standard deviation: 14.62; CV(%): 4.2 Sample 2: n=16; Mean(pg/ml): 1057; Standard deviation: 45.45; CV(%): 4.3 Sample 3: n=16; Mean(pg/ml): 2334; Standard deviation: 91.03; CV(%): 3.9

Sample 1: n=24; Mean(pg/ml): 361; Standard deviation: 21.3; CV(%): 5.9 Sample 2: n=24; Mean(pg/ml): 1049; Standard deviation: 67.14; CV(%): 6.4 Sample 3: n=24; Mean(pg/ml): 2645; Standard deviation: 140.2; CV(%): 5.3

There is no detectable cross-reactivity with other relevant proteins.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

This kit is used for quantitative detection of human ErbB-2 in cell culture supernates, serum, plasma(heparin, EDTA) and tissue homogenates.

Human

Catalog number Description Quantity - 96-well plate precoated with anti- human ErbB-2 antibody 1 ST0000-10 Lyophilized recombinant human ErbB-2 standard 10ng/tubex2 AR1107 Biotinylated anti- human ErbB-2 antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml

HER2/neu(also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340(cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22)2.

ERBB2

Receptor tyrosine-protein kinase erbB-2

v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

c erb B2 C erbB 2 CD340 CD340 antigen Cerb B2/neu protein CerbB2 Erb B2 erbb2 HER 2 HER 2/neu HER2 Her2/neu Herstatin Metastatic lymph node gene 19 protein MLN 19 MLN19 NEU NEU Proto Oncogene Neuro Glioblastoma Derived Oncogene Homolog Neuroblastoma/glioblastoma derived oncogene homolog NGL p185 ErbB2 p185erbB2 Proto oncogene c ErbB 2 Proto oncogene Neu Receptor protein tyrosine kinase ErbB2 precursor Receptor tyrosine protein kinase erbB 2 TKR1 Tyrosine kinase type cell surface receptor HER2 v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) Verb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog

P04626